Investigate every aspect of a cellular pathway from imaging of cell confluence and viability under different treatment conditions to quantitation of nucleic acids and protein to western blot analysis, a wealth of new knowledge is. The format of an input file or stream may be associated with a oemolstream using the setformat method, and may be retrieved with getformat. Molecular devices announce first microplate reader in space. Monitoring cytotoxicity on spectramax i3, minimax cytometer molecular devices author. Setup your microplate reader for optimal htrf readouts get on the list to receive remarkable content. The spectramax i3x multimode microplate reader measures spectralbased absorbance. Multimode microplate reader spectramax i3, molecular devices. Your total solution matched reagents, an imaging cytometer option, userupgradable. Arcturusxt microdissection instrument user guide 011209 rev.
Spectramax i3x fl1 validation protocol evelyn mcgown 20151012 20. Conjugatedoligomers the term conjugated means an alternation of multiple and single bonds linking a sequence of bonded atoms, such that there is an extended series of overlapping. Prepare data in the provided easytouse excel import templates import data into softmax pro from any non molecular devices microplate. Fluorescence polarization spectramax detection cartridge fluorescence polarization spectramax detection cartridges offers great sensitivity for spectramax i3 and paradigm systems. Following an introduction to the general concepts, the authors go on to discuss devices for processing electrons and electronic energy, memories, logic gates and related systems, and, finally, molecular. Spectramax m3 microplate reader from molecular devices llc description the spectramax m3 multimode microplate reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform.
Fluorophore list 2 on the desktop of the computer a pdf file if you have two fluorophores, you may have better separation of the two signals by using nonpeak wavelengths. Microplate spectrophotometer operators manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices corporation service facility. The absorbance at 490 nm was detected by spectramax i3 molecular devices, san jose, ca. Softmax pro data acquisition and analysis software. This is implemented in the formula system using the peakproanalysis operator, which can calculate sixteen different. Plate reader instructions biomolecular analysis facility. Sunnyvale, ca prweb molecular devices announced today the launch of the new spectramax id3 multimode microplate reader, a cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Automating scanlater western blot detection with stakmax. Molecular devices and machines the bottomup design, construction, and operation of devices and machines on the molecular scale is a topic of great interest in nanoscience and a fascinating challenge for nanotechnology. Apr 30, 20 inhibitors were added at concentrations between 0 and 10 m in kinase reaction buffer and tested in triplicate. If you like to customize your desktop, then this is the distro you you. The two different data import options are tailored to quickly get your data into softmax pro to provide a single source of software for all your data reduction needs. Catchpoint simplestep elisa spectramax i3 i3x cathy olsen 20180917 23. In vivo fluorescence imaging was made on a kodak invivo imaging system fx pro.
Superior flexibility the spectramax i3 multimode platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The spectramax i3x platform from molecular devices is a multimode microplate reader system that evolves with your future needs and offers an unlimited breadth of application possibilities. Technical note in the stakmax window that opens, select scripting and choose file path new to create a new script. If the plate is new, or has been recertified since the last calibration, the new absorbance values must. Although this user guide has been prepared with every precaution to ensure accuracy, molecular devices. The spectramax i3x multimode microplate reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Clinical and biochemical data of endothelial function in. Mitochondrial toxicity, spectramax minimax 300 cytometer.
Fluorescence imaging was conducted on an fv 1200ix83 confocal laser scanning microscope olympus. The spectramax gemini xs uses two holographic diffraction grating monochromators which allow for individual optimization of wavelengths for both excitation and emission. The percentage of cell viability was calculated by dividing the absorbance of the test group by the absorbance of the control group. Molecular devices is one of the leading provider of highperformance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. A few times the instrument did not work, because the wrong instrument selected in software. Fluorescence intensity spectramax paradigm or i3 detection. Supporting information elegant molecular iodineantisolvent. Molecular devices corporation reserves the right to change its products and services at any time to incorporate technological developments. After assessing parallelism of the doseresponse curves, s5 the sample binding relative to the reference standard was determined using graphpad prism graphpad software, san diego, california, usa with the 4. Explore how the spectramax minimax 300 imaging cytometer on the spectramax i3 multimode detection platform can be used to assess mitochondrial toxicity. A custom script was written to handle data file formatting, plate handling, data generation, and data storage. Using the molecular devices test plate to calibrate the spectramax reader. Gfp transfection with spectramax i3 multimode detection. Benralizumab for pdgfranegative hypereosinophilic syndrome.
Spectramax minimax 300 imaging cytometer molecular. Reactions were quenched with phosphoric acid, bound to 96well p81 phosphocellulose filter plates millipore and assayed with microscint 20 scintillation fluid perkin elmer using a spectramax l luminometer molecular devices. Molecular devices corporation reserv es the right to change its products and services at any time to incorporate technological developments. Cardiomyocyte beating i3 basic cathy olsen 20726 19. Molecular devices spectramax id3 multimode microplate. Spectramax m3, m4, m5, and m5e multimode microplate reader. Check your email, your application note is on the way. Robust, highvalue microplate readers can run samples based on predefined protocols and standard filter modes cover the entire visible range for a variety of assays. A arcturusxt microdissection instrument user guide molecular devices corporation 11 orleans drive sunnyvale, california 94089. These molecular systems, and a host of other equally diverse chemical species, are principal actors in the rapidly emerging field of molecular electronics. The molecular mechanism of ginsenoside analogs activating. Future ready the spectramax i3 x multimode microplate reader measures spectralbased absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors.
The trademarks mentioned herein are the property of molecular devices, llc or their. Scanlater western blot detection system molecular devices. The spectramax i3 x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. For a higher degree of automation, the automation vendor partners program has streamlined the integration of our microplate reader systems with all leading partner robots. Catchpoint simplestep elisa spectramax i3i3x softmax pro. This capabilities provide a tremendous new opportunity for. Our products enable scientists to improve productivity and efficiency in scientific discovery and research. Cardiomyocyte beating on spectramax i3, minimax imaging. Spectratest fl1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information.
This feature rich reader has a large, high resolution touchscreen with an. Let molecular devices and the spectramax i3 x multimode detection platform power your next landmark discovery. Software for the test plate must be installed on the control computer before the plate can be used. Cardiomyocyte beating i3 basic softmax pro protocol. The cell viability rate vr was calculated from the following equation. This document is in pdf format and can be viewed using a pdf viewer. Species made of interlocked molecular components are most attractive candidates for these purposes. Spectramax i3 multimode detection platform introduction expression of fluorescent proteins, for example, green fluorescent proteins gfp, is often used as a readout for reporter gene assays. Molecular devices corporation instrument warranty molecular devices corporation warrants this product against defects in material or workmanship as follows. Enabling the spectramax i3i3x and paradigm microplate readers antibodies designed to.
Molecular devices offers a wideranging portfolio of bioanalytical products and systems for life science research bioresearch. The spectramax i3 platform from molecular devices is a multimode detection system that evolves with your future needs and offers an unlimited breadth of. This user guide is subject to change without notice. Molecular devices spectramax id3 multimode microplate reader. Nanoantagonist alleviates inflammation and allows for mri of. Hardware user guides and then click the document you want to view.
Script setup in the operations tab of softmax pro software, plate stacker is selected. Spectramax i3 multimode platform molecular devices. Molecular devices cleanable reader assy cleanable reader assy. Spectramax m2m2e user guide university of michigan. System enables firstofitskind western blot membrane detection in. Spectramax i3 spectratest lm1 protocol softmax pro. The analyst, flexstation and spectramax m5m5 e from molecular devices have received the lanthascreen certified designation from life technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Molecular devices and machines article pdf available in physics world 1711.
Spectramax i3x fl1 validation protocol softmax pro. Spectratest lm1 this experiment contains this introduction, the certificate of calibration information certinfo, revision information. Spectramax microplate spectrophotometer operators manual. Molecular devices and machines a journey into the nano. The spectramax m3 multimode microplate reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Spectramax m3, m4, m5, and m5 e multimode microplate. Redox status in human erythrocytes and plasma was assayed using epr spectroscopy bruker xband epr spectrometer, emxmicro and colorimetric assays using spectramax i3 molecular devices, lls, usa. Creating interactive 3d pdf documents with vmd and acrobat3d. Fluorescence, and luminescence with the added functionality of.
Table 3 compatible fluorescence plate reader by manufacturer molecular devices bmg labtech. The trademarks used herein are the property of molecular devices, llc or their respective owners. New applications in minutes adding modes and functionality is just minutes away. After assessing parallelism of the doseresponse curves, the potency of test sample relative to the reference standard was determined using graphpad prism graphpad software with the 4 parameter logistic model. The energy needed to make a device work can be supplied as chemical energy, electrical energy, or light. A new class of small molecule inhibitor of bmp signaling. Molecular devices introduces spectramax id3 multimode. Htrf assays that utilize the tb donor with red d2 acceptor. Simply insert a cartridge to expand your application capabilities. With the spectramax minimax 300 imaging cytometer, industryleading softmax pro software, and user.
The dual photomultiplier tube design on spectramax paradigm accelerates the throughput 2x due to the direct excitation optical path. Molecular devices 03102040 threshold cleanable reader only. The molecular devices spectramax 250 microplate reader has an optical design that mimics a dualbeam spectrophotometer. In a typical reporter assay, the regulatory sequence of a gene of interest is attached to a reporter gene that can be easily detected and measured. Images were analyzed using molecular devices softmax pro 6. Spectratest fl1 this experiment contains the introduction this section, the certificate of calibration information certinfo, the. Microplate spectrophotometer operators manual iii this warranty only covers defects arising under normal usage and does not cover malfunctions of failures resulting from misuse, abuse, neglect, alteration, modification, or repairs by other than an authorized molecular devices cor.
Spectramax i3 spectratest lm1 protocol evelyn mcgown 20726 16. Spectramax gemini xs make it appropriate for applications within the. Spectratest instrument validation packages molecular devices. Each sample has a discrete sample and reference beam so that each well is measured directly.
Genepix pro software is a tool mainly used for biological experiments, and pieces of data gathered from these experiments are saved in files with the. The m5e reader includes industryleading softmax pro data analysis software that can meet all throughput, content, and multiplexing needs of researchers who conduct. Imaging of histological sections was conducted on a bx43 fluorescence microscope olympus. Softmax pro 7 data acquisition and analysis software. Bioglo reagent promega corporation and quantitated with a multimode reader molecular devices. We aim to give an impression of some of the different molecular electronic components and discuss the importance of molecular wires that should serve as interconnects between these devices. Spectramax m3 multimode microplate reader, molecular devices. Compoundinduced changes in observed beating rate can be correlated to cell viability for a more. Spectramax minimax 300 imaging cytometer molecular devices. Prepare data in the provided easytouse excel import templates import data into softmax pro from any nonmolecular devices microplate. Molecular devices software programs and related file extensions. Using the molecular devices test plate to calibrate the.
Researchers have created a variety of interesting molecular devices, including resonanttunneling diode rtd devices 4, 5, programmable molecular switches 6, carbon nanotube transistors, 7 and diodes 8, to name but a few. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96 or 384well microplate. Spectramax m3 microplate reader from molecular devices llc. Molecules have not historically played a prominent role in electronic devices. Spectramax i3 spectratest fl1 protocol evelyn mcgown 20726 16. The spectramax i3 platform from molecular devices is a multimode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. We also discuss the different approaches that are used. Spectramax m3, m4, m5, and m5e multimode microplate. The popular adobe pdf document format pdf version 1. The latest versions of adobe acrobat reader can now display, rotate, and animate 3d models embedded within these pdf documents. Spectramax i3x multimode microplate reader molecular devices. Setup molecular devices spectramax i3x tb green pdf. Ten years ago, chemical applications were limited to the use of small molecules such as. Molecular devices need energy to operate and signals to communicate with the operator.
Softmax pro software user guide marine biological laboratory. Spectramax i3x multi mode detection platform molecular devices. When connecting to the spectramax i3x and i3 multimode detection. Spectramax i3 spectratest fl1 protocol softmax pro.
Unparalleled performance on a personalized platform. This fieldupgradeable option with oneofakind brightfield cell segmentation, green. An ipone tb standard curve was run to assess performance of the spectramax i3 platform for. Nov 01, 2019 the organs were positioned on a lowfluorescence mat that was placed on a 96well plate insert of molecular devices i3 plate reader with a trf module wb cartridge and the plate was imaged using excitation of 340 nm and emission 615 nm. Molecular devices spectramax 250 microplate reader.